IntroductionIn this investigation I am investigating if varying the amount of sugar solution or distilled water determines the concentration of cell sap. I will do this by using different concentrations of sugar solution and distilled water. If the medium is hypotonic — a dilute solution, with a higher water concentration than the cell — the cell will gain water through osmosis. However if the medium is isotonic —a solution with exactly the same water concentration as the cell — there will be no net movement of water across the cell membrane. If the medium is hypertonic — a concentrated solution, with a lower water concentration than the cell — the cell will lose water by osmosis.Investigation variablesTo have a controlled reliable investigation I will control the following variables:To try my best to do the experiments at the same room temperature.I will prepare the carrots in the same way because any minor changes can ruin the experiment.